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Janus Probe

Doubling Recording Density

With microelectrodes on both sides of the probe shanks, DBC's new Janus probe can record from neurons hidden from traditional single-sided probes. These "blind spots" will always exist for a single-sided probe, regardless of channel count. Take another dive into the same brain region with the Janus probe and re-discover the underlying neural circuits with hidden single-units revealed.

Two Sides powerful.

The JANUS Probe

Delivers Results:
4469 Cells

In CA1

From 6 mice

Read the study
50%

Yield Increase*

over single-sided probes

See the designs
10

Recent Publications

Using Janus Probes

See them
* Typical Results

Key Publication

science-1400

NEUROSCIENCE

Selection of experience for memory by hippocampal sharp wave ripples

Wannan Yang, Chen Sun, Roman Huszár, Thomas Hainmueller, Kirill Kiselev, György Buzsáki

Abstract

Experiences need to be tagged during learning for further consolidation. However, neurophysiological mechanisms that select experiences for lasting memory are not known. By combining large-scale neural recordings in mice with dimensionality reduction techniques, we observed that successive maze traversals were tracked by continuously drifting populations of neurons, providing neuronal signatures of both places visited and events encountered. When the brain state changed during reward consumption, sharp wave ripples (SPW-Rs) occurred on some trials, and their specific spike content decoded the trial blocks that surrounded them. During postexperience sleep, SPW-Rs continued to replay those trial blocks that were reactivated most frequently during waking SPW-Rs. Replay content of awake SPW-Rs may thus provide a neurophysiological tagging mechanism to select aspects of experience that are preserved and consolidated for future use.

The authors have described their work and analysis here: https://winnieyangwannan.github.io/RippleTagging/

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"Being able to record from large numbers of neurons simultaneously was really crucial for our study. Dual side probe from Diagnostic Biochips was really the key for collecting the high quality data used in this study."

Winnie Yang, NYU Neuroscience Institute & New York University, Langone Medical Center

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Single-units from CA1 Cell Layer Recorded from Both Sides of the Probe Shanks 

Isolated pyramidal cells (triangles) and interneurons (circles) from microelectrodes (squares) on both front (red) and back (blue) sides of probe shanks.

 

Cell Map

A Symphony from the Hippocampus

A symphony orchestrated by 422 pyramidal cells from CA1 of a mouse recorded during a spatial navigation task. Each cell corresponds to a specific note of a particular instrument. 

Can you spot place cell sequences during exploration and two ripples while the animal is at rest? 

Courtesy of the Buzsáki lab